CoraLite® Plus 488-conjugated DKK3 Monoclonal antibody

DKK3 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4E6H6

Cat no : CL488-66758

Synonyms

Dickkopf 3, Dickkopf related protein 3, Dkk 3, DKK3, hDkk 3, REIC, RIG

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Validation Data Gallery

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  IF Staining of HEK-293 using CL488-66758

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite®®488 DKK3 antibody (CL488-66758, Clone: 4E6H6 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66758 targets DKK3 in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: DKK3 fusion protein Ag23615
• Full Name: dickkopf homolog 3 (Xenopus laevis)
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 40 kDa, 50-55 kDa
• GenBank Accession Number: BC007660
• Gene Symbol: DKK3
• Gene ID (NCBI): 27122
• RRID: AB_2919363
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DKK3, also named as REIC, belongs to the dickkopf family. It antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer's disea. The adenovirus carrying REIC/Dkk-3 (Ad-REIC) exhibits a potent tumor-specific cell-killing function for various human cancers. It has also become evident that some human cancers are resistant to Ad-REIC-induced apoptosis. The DKK3 is a Glycosylation protein with MW about 55 kDa or 38-43 kDa.