DICER1 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

DICER1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 20567-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HepG2 using 20567-1-AP (same clone as 20567-1-PBS)

  WB result of DICER1 antibody (20567-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-DICER1 transfected HepG2 cells. This data was developed using the same antibody clone with 20567-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 20567-1-AP (same clone as 20567-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20567-1-AP (DICER1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20567-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 20567-1-AP (same clone as 20567-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 20567-1-AP (DICER1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20567-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 20567-1-AP (same clone as 20567-1-PBS)

  IP result of anti-DICER1 (IP:20567-1-AP, 5ug; Detection:20567-1-AP 1:300) with Jurkat cells lysate 2000ug. This data was developed using the same antibody clone with 20567-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 20567-1-AP (same clone as 20567-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20567-1-AP (DICER1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 20567-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 20567-1-AP (same clone as 20567-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20567-1-AP (DICER1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20567-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 20567-1-AP (same clone as 20567-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20567-1-AP (DICER1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 20567-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 20567-1-AP (same clone as 20567-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20567-1-AP (DICER1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20567-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 20567-1-AP (same clone as 20567-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20567-1-AP (DICER1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20567-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 20567-1-AP (same clone as 20567-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20567-1-AP (DICER1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20567-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human ovary tumor using 20567-1-AP (same clone as 20567-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using DICER1 antibody (20567-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20567-1-PBS in a different storage buffer formulation.

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  IF Staining of human ovary tumor using 20567-1-AP (same clone as 20567-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using DICER1 antibody (20567-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20567-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 20567-1-AP (same clone as 20567-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DICER1 antibody (20567-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20567-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 20567-1-AP (same clone as 20567-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human DICER1 (20567-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20567-1-PBS in a different storage buffer formulation.

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Product Information

20567-1-PBS targets DICER1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: dicer 1, ribonuclease type III
• Calculated Molecular Weight: 219 kDa
• Observed Molecular Weight: 220-250 kDa, 90 kDa
• GenBank Accession Number: NM_030621
• Gene Symbol: DICER1
• Gene ID (NCBI): 23405
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DICER1, also named as DICER, HERNA and KIAA0928, belongs to the helicase family and Dicer subfamily. It is required for formation of the RNA induced silencing complex (RISC). DICER1 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. DICER1 cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs. The antibody is specific to DICER1. The calcualted molecular weight of DICER1 is 219 kDa, but modified DICER1 is about 220-250 kDa. DICER1 exists some isoforms with molecular weight 219 kDa and 93 kDa.