DHX9 Monoclonal antibody, PBS Only (Detector)

• Featured Product
• KD/KO Validated

DHX9 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B12C10

Cat no : 67153-1-PBS

Synonyms

ATP dependent RNA helicase A, DDX9, DEAH box protein 9, DHX9, LKP, NDH II, NDH2, NDHII, Nuclear DNA helicase II, RHA

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Validation Data Gallery

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  WB analysis using 67153-1-Ig (same clone as 67153-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67153-1-Ig (same clone as 67153-1-PBS)

  WB result of DHX9 antibody (67153-1-Ig; 1:400000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHX9 transfected HEK-293 cells. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67153-1-Ig (same clone as 67153-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67153-1-Ig (same clone as 67153-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67153-1-Ig (same clone as 67153-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67153-1-Ig (same clone as 67153-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 67153-1-Ig (same clone as 67153-1-PBS)

  IP result of anti-DHX9 (IP:67153-1-Ig, 5ug; Detection:67153-1-Ig 1:20000) with HeLa cells lysate 2000 ug. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67153-1-Ig (same clone as 67153-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67153-1-Ig (DHX9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67153-1-Ig (same clone as 67153-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67153-1-Ig (DHX9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67153-1-Ig (same clone as 67153-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67153-1-Ig (DHX9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67153-1-Ig (same clone as 67153-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67153-1-Ig (DHX9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67153-1-Ig (same clone as 67153-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67153-1-Ig (DHX9 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50161-1

  Cytometric bead array standard curve of MP50161-1, DHX9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67153-2-PBS. Detection antibody: 67153-1-PBS. Standard:Ag12104. Range: 1.563-100 ng/mL.

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Product Information

67153-1-PBS targets DHX9 as part of a matched antibody pair:

MP50161-1: 67153-2-PBS capture and 67153-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DHX9 fusion protein Ag12104
• Full Name: DEAH (Asp-Glu-Ala-His) box polypeptide 9
• Calculated Molecular Weight: 1270 aa, 141 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC014246
• Gene Symbol: DHX9
• Gene ID (NCBI): 1660
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RNA helicases play important roles in transcription, RNA processing, translation, and RNA replication. DEAD box proteins are putative RNA helicases that have a characteristic Asp-Glu-Ala-Asp (DEAD) box as 1 of 8 highly conserved sequence motifs. DHX9 a member of the DEAH family of proteins, which possess a double-stranded RNA-binding domain (dsRBD) and a helicase domain [PMID:20569003]. It unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure of DHX9 may subsequently influence interactions with proteins or other nucleic acids. It is also a component of the CRD-mediated complex that promotes MYC mRNA stability. In addition, it is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2[PMID: 19029303, 22190748].