• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

DHX9 Monoclonal antibody

DHX9 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

CloneNo.

1B12C10

Cat no : 67153-1-Ig

Synonyms

ATP dependent RNA helicase A, DDX9, DEAH box protein 9, DHX9, LKP, NDH II, NDH2, NDHII, Nuclear DNA helicase II, RHA

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using DHX9 Monoclonal antibody (67153-1-Ig)

    WB analysis using 67153-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • Western Blot (WB) analysis of HEK-293 cells using DHX9 Monoclonal antibody (67153-1-Ig)

    WB analysis of HEK-293 using 67153-1-Ig

    WB result of DHX9 antibody (67153-1-Ig; 1:400000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHX9 transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using DHX9 Monoclonal antibody (67153-1-Ig)

    WB analysis using 67153-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using DHX9 Monoclonal antibody (67153-1-Ig)

    WB analysis of HepG2 using 67153-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using DHX9 Monoclonal antibody (67153-1-Ig)

    WB analysis of HEK-293 using 67153-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DHX9 Monoclonal antibody (67153-1-Ig)

    WB analysis of HeLa using 67153-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using DHX9 Monoclonal antibody (67153-1-Ig)

    IP experiment of HeLa using 67153-1-Ig

    IP result of anti-DHX9 (IP:67153-1-Ig, 5ug; Detection:67153-1-Ig 1:20000) with HeLa cells lysate 2000 ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DHX9 Monoclonal antibody (67153-1-Ig)

    IHC staining of mouse brain using 67153-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67153-1-Ig (DHX9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DHX9 Monoclonal antibody (67153-1-Ig)

    IHC staining of human breast cancer using 67153-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67153-1-Ig (DHX9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DHX9 Monoclonal antibody (67153-1-Ig)

    IHC staining of human breast cancer using 67153-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67153-1-Ig (DHX9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DHX9 Monoclonal antibody (67153-1-Ig)

    IHC staining of mouse brain using 67153-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67153-1-Ig (DHX9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DHX9 Monoclonal antibody (67153-1-Ig)

    IF Staining of HepG2 using 67153-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67153-1-Ig (DHX9 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, THP-1 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67153-1-Ig targets DHX9 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DHX9 fusion protein Ag12104
Full Name DEAH (Asp-Glu-Ala-His) box polypeptide 9
Calculated Molecular Weight 1270 aa, 141 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC014246
Gene Symbol DHX9
Gene ID (NCBI) 1660
RRIDAB_2882450
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNA helicases play important roles in transcription, RNA processing, translation, and RNA replication. DEAD box proteins are putative RNA helicases that have a characteristic Asp-Glu-Ala-Asp (DEAD) box as 1 of 8 highly conserved sequence motifs. DHX9 a member of the DEAH family of proteins, which possess a double-stranded RNA-binding domain (dsRBD) and a helicase domain [PMID:20569003]. It unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure of DHX9 may subsequently influence interactions with proteins or other nucleic acids. It is also a component of the CRD-mediated complex that promotes MYC mRNA stability. In addition, it is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2[PMID: 19029303, 22190748].

Protocols

Product Specific Protocols
WB protocol for DHX9 antibody 67153-1-IgDownload protocol
IHC protocol for DHX9 antibody 67153-1-IgDownload protocol
IF protocol for DHX9 antibody 67153-1-IgDownload protocol
IP protocol for DHX9 antibody 67153-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

Sci Adv

Nucleic DHX9 cooperates with STAT1 to transcribe interferon-stimulated genes

Authors - Xingxing Ren
View Article
humanWB

Front Oncol

MS0621, a novel small-molecule modulator of Ewing sarcoma chromatin accessibility, interacts with an RNA-associated macromolecular complex and influences RNA splicing

Authors - Tamara Vital
View Article
mouseIP,RIP,WB,IF,CoIP

Cell Rep

Virus-induced lncRNA-BTX allows viral replication by regulating intracellular translocation of DHX9 and ILF3 to induce innate escape

Authors - Yang Cao
View Article
humanIF

Nat Commun

DHX9 maintains epithelial homeostasis by restraining R-loop-mediated genomic instability in intestinal stem cells

Authors - Xingxing Ren
View Article
humanWB

J Am Chem Soc

Polymerase η Recruits DHX9 Helicase to Promote Replication across Guanine Quadruplex Structures

Authors - Feng Tang
View Article