DHX32 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

DHX32 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

2A4G9

Cat no : 68306-1-PBS

Synonyms

HuDDX32, EC:3.6.4.13, DHLP1, DEAD/H helicase-like protein 1, DDX32

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Validation Data Gallery

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  WB analysis using 68306-1-Ig (same clone as 68306-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68306-1-Ig (DHX32 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68306-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 68306-1-Ig (same clone as 68306-1-PBS)

  WB result of DHX32 antibody (68306-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHX32 transfected A549 cells. This data was developed using the same antibody clone with 68306-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68306-1-Ig (same clone as 68306-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68306-1-Ig (DHX32 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68306-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U2OS using 68306-1-Ig (same clone as 68306-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 68306-1-Ig (DHX32 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68306-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68306-1-Ig (same clone as 68306-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68306-1-Ig (DHX32 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68306-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 68306-1-Ig (same clone as 68306-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug DHX32 Monoclonal antibody (68306-1-Ig, Clone:2A4G9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68306-1-PBS in a different storage buffer formulation.

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Product Information

68306-1-PBS targets DHX32 in Indirect ELISA, WB applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: DHX32 fusion protein Ag13956
• Full Name: DEAH (Asp-Glu-Ala-His) box polypeptide 32
• Calculated Molecular Weight: 743 aa, 84 kDa
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC002473
• Gene Symbol: DHX32
• Gene ID (NCBI): 55760
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DHX32, an RNA helicase, belongs to the DEAH box RNA helicase family by virtue of its homology to the conserved helicase domain. DHX32 is abundantly expressed in human and murine models, participating in cell transcription and differentiation, and being capable of regulating T-cell apoptosis. DHX32 is also associated with some malignant tumors, such as lymphoma, colorectal cancer, and breast cancer (PMID: 35117530). DHX32 has 2 isoforms with molecular weights of 75 and 84 kDa, respectively.