DHX15 Recombinant antibody, PBS Only

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  WB analysis of HeLa using 82137-1-RR (same clone as 82137-1-PBS)

  WB result of DHX15 antibody (82137-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHX15 transfected HeLa cells. This data was developed using the same antibody clone with 82137-1-PBS in a different storage buffer formulation.

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  WB analysis using 82137-1-RR (same clone as 82137-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82137-1-RR (DHX15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82137-1-PBS in a different storage buffer formulation.

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  IP experiment of NIH/3T3 using 82137-1-RR (same clone as 82137-1-PBS)

  IP result of anti-DHX15 (IP:82137-1-RR, 4ug; Detection:82137-1-RR 1:8000) with NIH/3T3 cells lysate 1320 ug. This data was developed using the same antibody clone with 82137-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 82137-1-RR (same clone as 82137-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82137-1-RR (DHX15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82137-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 82137-1-RR (same clone as 82137-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82137-1-RR (DHX15 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82137-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82137-1-RR (same clone as 82137-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DHX15 antibody (82137-1-RR, Clone: 1F13 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82137-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82137-1-RR (same clone as 82137-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DHX15 antibody (82137-1-RR, Clone: 1F13 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82137-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82137-1-RR (same clone as 82137-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DHX15 antibody (82137-1-RR, Clone: 1F13 ) at dilution of 1:2500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 82137-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82137-1-RR (same clone as 82137-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DHX15 (82137-1-RR, Clone:1F13) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82137-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82137-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82137-1-RR against Human DHX15 were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

DHX15 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Cat No. 82137-1-PBS

Clone No.1F13

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

EC:3.6.4.13, DDX15, DBP1, ATP-dependent RNA helicase DHX15, ATP-dependent RNA helicase #46

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Product Information

82137-1-PBS targets DHX15 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DHX15 fusion protein Ag2895
• Full Name: DEAH (Asp-Glu-Ala-His) box polypeptide 15
• Calculated Molecular Weight: 795 aa, 91 kDa
• Observed Molecular Weight: 90-95 kDa
• GenBank Accession Number: BC035974
• Gene Symbol: DHX15
• Gene ID (NCBI): 1665
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O43143
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DHX15, also named as DBP1 and DDX15, is a DExD/H-box protein that has been identified in spliceosome, through its carboxyl (C)-terminal G-patch domain and stimulates the helicase activity of DHX15. DHX15 is a pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. The MW of DHX15 is about 90-95kDa.