DHRS2 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

DHRS2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15735-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of SKOV-3 using 15735-1-AP (same clone as 15735-1-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 15735-1-AP (DHRS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15735-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 15735-1-AP (same clone as 15735-1-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 15735-1-AP (DHRS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15735-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 15735-1-AP (same clone as 15735-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 15735-1-AP (DHRS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15735-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 15735-1-AP (same clone as 15735-1-PBS)

  IP result of anti-DHRS2 (IP:15735-1-AP, 4ug; Detection:15735-1-AP 1:300) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 15735-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15735-1-AP (same clone as 15735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15735-1-AP (DHRS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15735-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 15735-1-AP (same clone as 15735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15735-1-AP (DHRS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15735-1-PBS in a different storage buffer formulation.

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Product Information

15735-1-PBS targets DHRS2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DHRS2 fusion protein Ag8508
• Full Name: dehydrogenase/reductase (SDR family) member 2
• Calculated Molecular Weight: 300 aa, 32 kDa
• Observed Molecular Weight: 27-30 kDa
• GenBank Accession Number: BC007339
• Gene Symbol: DHRS2
• Gene ID (NCBI): 10202
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DHRS2(Dehydrogenase/reductase SDR family member 2) is also named as dicarbonyl reductase HEP27, Protein D and belongs to the short-chain dehydrogenases/reductases (SDR) family. It displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2-propanedione as substrates and there is no reductase activity displayed in vitro with steroids, retinoids and sugars as substrates. This protein also may inhibit cell replication. It has 2 isoforms produced by alternative splicing with the molecular weight of 27 kDa and 29 kDa.