DHRS2 Polyclonal antibody

• Featured Product
• KD/KO Validated

DHRS2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 15735-1-AP

Synonyms

DHRS2, Dicarbonyl reductase HEP27, HEP27, Protein D, SDR25C1

---

Validation Data Gallery

• 

  WB analysis of SKOV-3 using 15735-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 15735-1-AP (DHRS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15735-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 15735-1-AP (DHRS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 15735-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15735-1-AP (DHRS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15735-1-AP

  IP result of anti-DHRS2 (IP:15735-1-AP, 4ug; Detection:15735-1-AP 1:300) with HeLa cells lysate 4000ug.

• 

  IHC staining of human liver cancer using 15735-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15735-1-AP (DHRS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15735-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15735-1-AP (DHRS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: SKOV-3 cells, human liver tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Product Information

15735-1-AP targets DHRS2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DHRS2 fusion protein Ag8508
• Full Name: dehydrogenase/reductase (SDR family) member 2
• Calculated Molecular Weight: 300 aa, 32 kDa
• Observed Molecular Weight: 27-30 kDa
• GenBank Accession Number: BC007339
• Gene Symbol: DHRS2
• Gene ID (NCBI): 10202
• RRID: AB_2091847
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHRS2(Dehydrogenase/reductase SDR family member 2) is also named as dicarbonyl reductase HEP27, Protein D and belongs to the short-chain dehydrogenases/reductases (SDR) family. It displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2-propanedione as substrates and there is no reductase activity displayed in vitro with steroids, retinoids and sugars as substrates. This protein also may inhibit cell replication. It has 2 isoforms produced by alternative splicing with the molecular weight of 27 kDa and 29 kDa.

Publications

Species	Application	Title
human	WB,IHC	Epigenetics; Decreased DHRS2 expression is associated with HDACi resistance and poor prognosis in ovarian cancer.; Authors - Yingyan Han ; KD Validated
human	WB	Oncol Rep; PMID: 31436301; Authors - Ji-Min Li ; KD Validated
human	WB	Cell Death Dis; REV1 promotes lung tumorigenesis by activating the Rad18/SERTAD2 axis.; Authors - Yunshang Chen
human	WB	Transl Oncol; STAMBPL1 promotes the progression of lung adenocarcinoma by inhibiting DHRS2 expression; Authors - Xiang Yang
human	IHC	Iran J Public Health; Auxiliary Diagnosis and Prognostic Value of Dehydrogenase/Reductase 2 (DHRS2) in Various Tumors; Authors - Zhongwu An