DGAT1 Recombinant antibody

• Featured Product
• KD/KO Validated

DGAT1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230091E1

Cat no : 82945-1-RR

Synonyms

ACAT related gene product 1, AGRP1, ARGP1, DGAT, DGAT1, Diglyceride acyltransferase

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Validation Data Gallery

• 

  WB analysis using 82945-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82945-1-RR (DGAT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 82945-1-RR

  WB result of DGAT1 antibody (82945-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DGAT1 transfected HeLa cells.

• 

  WB analysis using 82945-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82945-1-RR (DGAT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue, HepG2 cells, PC-3 cells, THP-1 cells, Caco-2 cells, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82945-1-RR targets DGAT1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DGAT1 fusion protein Ag32717
• Full Name: diacylglycerol O-acyltransferase homolog 1 (mouse)
• Calculated Molecular Weight: 488 aa, 55 kDa
• Observed Molecular Weight: 45-55 kDa
• GenBank Accession Number: BC015762
• Gene Symbol: DGAT1
• Gene ID (NCBI): 8694
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.