Desmin Monoclonal antibody

Desmin Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

9

CloneNo.

3E12C8

Cat no : 60226-1-Ig

Synonyms

CMD1I, CSM1, CSM2, DES, desmin

---

Validation Data Gallery

• 

  WB analysis using 60226-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60226-1-Ig (Desmin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 60226-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 60226-1-Ig (DES antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: Human heart, human testis tissue, human heart tissue, human placenta tissue, pig heart tissue, pig colon tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

60226-1-Ig targets Desmin in WB, IF, IHC, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Desmin fusion protein Ag9675
• Full Name: desmin
• Calculated Molecular Weight: 470 aa, 54 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC032116
• Gene Symbol: Desmin
• Gene ID (NCBI): 1674
• RRID: AB_11042773
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.

Publications

Species	Application	Title
mouse	WB	Br J Pharmacol; Catalpol Alleviates Adriamycin-Induced Nephropathy by Activating the SIRT1 Signalling Pathway In Vivo and In Vitro.; Authors - Jiangnan Zhang
rat	IF	Diabetes Metab Res Rev; Gene expression profiles of rat MMECs with different glucose levels and fgl2 gene silencing.; Authors - Zhenzhong Zheng
mouse	IHC	Exp Cell Res; Effect of sarcolipin-mediated cell transdifferentiation in sarcopenia-associated skeletal muscle fibrosis.; Authors - Qiu-Nan Chen
human	IF	Exp Eye Res; Botox-A improve the thyroid-associated ophthalmopathy (TAO) orbital fibroblast activation through inhibiting the TGF-β/Smad signaling.; Authors - Xin Qi
human	IF	Reprod Biomed Online; Stomatin-like protein 2 is involved in endometrial stromal cell proliferation and differentiation during decidualization in mice and humans.; Authors - Qian Feng
mouse	WB	BMC Nephrol; Adiponectin protects obesity-related glomerulopathy by inhibiting ROS/NF-κB/NLRP3 inflammation pathway.; Authors - Xiaohong Xu