DEK Recombinant antibody, PBS Only

• Featured Product
• KD/KO Validated

DEK Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230065H2

Cat no : 82880-1-PBS

Synonyms

D6S231E, 230065H2

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Validation Data Gallery

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  WB analysis of HEK-293 using 82880-1-RR (same clone as 82880-1-PBS)

  WB result of DEK antibody (82880-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DEK transfected HEK-293 cells. This data was developed using the same antibody clone with 82880-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 82880-1-RR (same clone as 82880-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82880-1-RR (DEK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82880-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 82880-1-RR (same clone as 82880-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82880-1-RR (DEK antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82880-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 82880-1-RR (same clone as 82880-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DEK antibody (82880-1-RR, Clone: 230065H2 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82880-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82880-1-RR (same clone as 82880-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DEK antibody (82880-1-RR, Clone: 230065H2 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82880-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 82880-1-RR (same clone as 82880-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug DEK Recombinant antibody (82880-1-RR, Clone:230065H2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82880-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82880-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82880-1-RR against Human DEK were performed. The affinity constant is below 1 pM.

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Product Information

82880-1-PBS targets DEK in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DEK fusion protein Ag8225
• Full Name: DEK oncogene
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC035259
• Gene Symbol: DEK
• Gene ID (NCBI): 7913
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Dek oncogene(DEK) is first recognized in a fusion with CAN nucleoporin protein, and it's a also autoantigen in patients with pquciarticular onset juvenile rheumatoid arthritis. As a site-specific binding protein, DEK may involve in transcriptional regulation and signal transdcution. Analysis by agarose gel celctrophoresis, DEK showed to reduce the efficiency with which chromation is replicated. It's one of the first components of the splicing complex, which remains associate with spliced exons dependent on prior splicing of pre-mRNA.