DEFA1 Monoclonal antibody, PBS Only

DEFA1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A2B3

Cat no : 67156-1-PBS

Synonyms

DEF1, DEFA1, DEFA1B, DEFA2, defensin, alpha 1, HNP 1, HNP 2, HP 1, HP 2, HP1, HP2, MRS, Neutrophil defensin 1

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Validation Data Gallery

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  WB analysis of human peripheral blood leukocyte using 67156-1-Ig (same clone as 67156-1-PBS)

  Human peripheral blood leukocyte cells were subjected to SDS PAGE followed by western blot with 67156-1-Ig (DEFA1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67156-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 67156-1-Ig (same clone as 67156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67156-1-Ig (DEFA1 antibody) at dilution of 1:200 (under 10x lens).. This data was developed using the same antibody clone with 67156-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 67156-1-Ig (same clone as 67156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67156-1-Ig (DEFA1 antibody) at dilution of 1:200 (under 40x lens).. This data was developed using the same antibody clone with 67156-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 67156-1-Ig (same clone as 67156-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using DEFA1 antibody (67156-1-Ig, Clone: 1A2B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67156-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67156-1-PBS targets DEFA1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: DEFA1 fusion protein Ag12592
• Full Name: defensin, alpha 1
• Calculated Molecular Weight: 94 aa, 10 kDa
• Observed Molecular Weight: 6-10 kDa
• GenBank Accession Number: BC093791
• Gene Symbol: DEFA1
• Gene ID (NCBI): 1667
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.