DEFA1 Monoclonal antibody
DEFA1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
1A2B3
Cat no : 67156-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Human peripheral blood leukocyte cells |
Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse spleen tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
67156-1-Ig targets DEFA1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | DEFA1 fusion protein Ag12592 |
Full Name | defensin, alpha 1 |
Calculated Molecular Weight | 94 aa, 10 kDa |
Observed Molecular Weight | 6-10 kDa |
GenBank Accession Number | BC093791 |
Gene Symbol | DEFA1 |
Gene ID (NCBI) | 1667 |
RRID | AB_2882453 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for DEFA1 antibody 67156-1-Ig | Download protocol |
IHC protocol for DEFA1 antibody 67156-1-Ig | Download protocol |
IF protocol for DEFA1 antibody 67156-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |