• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

DEFA1 Monoclonal antibody

DEFA1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1A2B3

Cat no : 67156-1-Ig

Synonyms

HNP-1, defensin, alpha 1, DEFA2, DEFA1B, DEFA 1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human peripheral blood leukocyte using DEFA1 Monoclonal antibody (67156-1-Ig)

    WB analysis of human peripheral blood leukocyte using 67156-1-Ig

    Human peripheral blood leukocyte cells were subjected to SDS PAGE followed by western blot with 67156-1-Ig (DEFA1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using DEFA1 Monoclonal antibody (67156-1-Ig)

    IHC staining of human small intestine using 67156-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67156-1-Ig (DEFA1 antibody) at dilution of 1:2000 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using DEFA1 Monoclonal antibody (67156-1-Ig)

    IHC staining of human small intestine using 67156-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67156-1-Ig (DEFA1 antibody) at dilution of 1:2000 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using DEFA1 Monoclonal antibody (67156-1-Ig)

    IF Staining of mouse spleen using 67156-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using DEFA1 antibody (67156-1-Ig, Clone: 1A2B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHuman peripheral blood leukocyte cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67156-1-Ig targets DEFA1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen DEFA1 fusion protein Ag12592
Full Name defensin, alpha 1
Calculated Molecular Weight 94 aa, 10 kDa
Observed Molecular Weight 6-10 kDa
GenBank Accession NumberBC093791
Gene Symbol DEFA1
Gene ID (NCBI) 1667
RRIDAB_2882453
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DEFA1 antibody 67156-1-IgDownload protocol
IHC protocol for DEFA1 antibody 67156-1-IgDownload protocol
IF protocol for DEFA1 antibody 67156-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols