RIG-1/DDX58 Monoclonal antibody

RIG-1/DDX58 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

3B10F5

Cat no : 67556-1-Ig

Synonyms

DDX58, RIG-I, EC:3.6.4.13, DEAD box protein 58, ATP-dependent RNA helicase DDX58

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Validation Data Gallery

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  WB analysis using 67556-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67556-1-Ig (DDX58 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 67556-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RIG-1/DDX58 antibody (67556-1-Ig, Clone: 3B10F5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, Hela cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

67556-1-Ig targets RIG-1/DDX58 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RIG-1/DDX58 fusion protein Ag18591
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
• Calculated Molecular Weight: 925 aa, 106 kDa
• Observed Molecular Weight: 101/106 kDa
• GenBank Accession Number: BC132786
• Gene Symbol: RIG-1/DDX58
• Gene ID (NCBI): 23586
• RRID: AB_2882770
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. The antibody is specific to DDX58,which has two isoform with the MW of 106 and 101 kDa.

Publications

Species	Application	Title
human	IF	Autophagy; N protein of PEDV plays chess game with host proteins by selective autophagy; Authors - Xueying Zhai
		J Virol; RBM14 inhibits the replication of porcine epidemic diarrhea virus by recruiting p62 to degrade nucleocapsid protein through the activation of autophagy and interferon pathway; Authors - Xiaoquan Wang
human	WB	Nat Commun; TRIM25 predominately associates with anti-viral stress granules; Authors - Zehua Shang
human	WB	Redox Biol; Nlrp6 protects from corticosterone-induced NSPC ferroptosis by modulating RIG-1/MAVS-mediated mitophagy; Authors - Jingyan Shen