DDX21 Monoclonal antibody, PBS Only

DDX21 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B9C8

Cat no : 66925-1-PBS

Synonyms

GUA, Gu alpha, EC:3.6.4.13, DExD-box helicase 21, DEAD box protein 21

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Validation Data Gallery

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  WB analysis using 66925-1-Ig (same clone as 66925-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66925-1-Ig (DDX21 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66925-1-PBS in a different storage buffer formulation.

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  WB analysis using 66925-1-Ig (same clone as 66925-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66925-1-Ig (DDX21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66925-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66925-1-Ig (same clone as 66925-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66925-1-Ig (DDX21 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66925-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66925-1-Ig (same clone as 66925-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66925-1-Ig (DDX21 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66925-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66925-1-Ig (same clone as 66925-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66925-1-Ig (DDX21 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66925-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66925-1-Ig (same clone as 66925-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66925-1-Ig (DDX21 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66925-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66925-1-Ig (same clone as 66925-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX21 antibody (66925-1-Ig, Clone: 1B9C8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66925-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66925-1-Ig (same clone as 66925-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human DDX21 (66925-1-Ig, Clone:1B9C8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66925-1-PBS in a different storage buffer formulation.

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Product Information

66925-1-PBS targets DDX21 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DDX21 fusion protein Ag17850
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 21
• Calculated Molecular Weight: 783 aa, 87 kDa
• Observed Molecular Weight: 90-95 kDa
• GenBank Accession Number: BC008071
• Gene Symbol: DDX21
• Gene ID (NCBI): 9188
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DDX21 protein belongs to DEAD box protein family which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). As a putative RNA helicase, DDX21 unwinds double-stranded RNA, folds single-stranded RNA and is involved in process including ribosomal RNA biogeneis, RNA editing and general transcription. Interaction of DDX21 and c-Jun was reported in ribosomal RNA processing.