DDR1 Recombinant antibody, PBS Only (Detector)

DDR1 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241796G1

Cat no : 84438-3-PBS

Synonyms

Cell adhesion kinase, CD167a, CD167 antigen-like family member A, CD167, CAK

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP01290-4

  Sandwich ELISA standard curve of MP01290-4, Humam DDR1 Recombinant Matched Antibody Pair - PBS only. 84438-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1675. 84438-3-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL

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  Sample test of MP01290-4

  MCF-7 human breast cancer cells (5 x 10^6 cells/mL) were cultured in DMEM supplemented with 10% fetal bovine serum, 4 mM L-glutamine, 4500mg/L glucose, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human DDR1, and measured 19.81 ng/mL

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  Sample test of MP01290-4

  The mean DDR1 concentration was determined to be 9.28 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load.

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  Sample test of MP01290-4

  Serum of four individual healthy human donors was measured. The human DDR1 concentration of detected samples was determined to be 13.63 ng/mL with a range of 9.29 - 18.03 ng/mL.

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  Cytometric bead array standard curve of MP01290-1

  Cytometric bead array standard curve of MP01290-1, DDR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84438-2-PBS. Detection antibody: 84438-3-PBS. Standard: Eg1675. Range: 0.313-40 ng/mL.

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  Cytometric bead array standard curve of MP01290-3

  Cytometric bead array standard curve of MP01290-3, DDR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84438-4-PBS. Detection antibody: 84438-3-PBS. Standard: Eg1675. Range: 0.313-40 ng/mL.

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Product Information

84438-3-PBS targets DDR1 as part of a matched antibody pair:

MP01290-1: 84438-2-PBS capture and 84438-3-PBS detection (validated in Cytometric bead array)

MP01290-3: 84438-4-PBS capture and 84438-3-PBS detection (validated in Cytometric bead array)

MP01290-4: 84438-5-PBS capture and 84438-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FusionProtein
• Full Name: discoidin domain receptor tyrosine kinase 1
• Calculated Molecular Weight: 101kDa
• GenBank Accession Number: NM_001297654.2
• Gene Symbol: DDR1
• Gene ID (NCBI): 780
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.