DCLK1 Polyclonal antibody, PBS Only

DCLK1 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29800-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 29800-1-AP (same clone as 29800-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29800-1-AP (DCLK1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 29800-1-AP (same clone as 29800-1-PBS)

  IP result of anti-DCLK1 (IP:29800-1-AP, 4ug; Detection:29800-1-AP 1:50000) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29800-1-AP (same clone as 29800-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29800-1-AP (same clone as 29800-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 29800-1-AP (same clone as 29800-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 29800-1-AP (same clone as 29800-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 29800-1-AP (same clone as 29800-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DCLK1 antibody (29800-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 29800-1-AP (same clone as 29800-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DCLK1 antibody (29800-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

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Product Information

29800-1-PBS targets DCLK1 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DCLK1 fusion protein Ag32007
• Full Name: doublecortin-like kinase 1
• Calculated Molecular Weight: 82KD
• Observed Molecular Weight: 48 kDa, 82 kDa
• GenBank Accession Number: NM_004734
• Gene Symbol: DCLK1
• Gene ID (NCBI): 9201
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DCLK1 (Serine/threonine-protein kinase DCLK1) is also named as DCAMKL1, DCDC3A, KIAA0369 and belongs to the CAMK Ser/Thr protein kinase family. It is a microtubule-associated kinase that can undergo autophosphorylation and it also has microtubule-polymerizing activity that is independent of its protein kinase activity (PMID: 11124993). It plays a unique role in mitotic spindle integrity during early neurogenesis in radial glial cell proliferation and their radial process stability. DCLK1 is a unique marker for distinguishing tumor stem cells from intestinal normal stem cells (PMID: 23202126). This protein has 4 isoforms produced by alternative splicing with the molecular weight of 82 kDa, 81 kDa, 47 kDa and 48 kDa.