Various lysates were subjected to SDS PAGE followed by western blot with 29800-1-AP (DCLK1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 29800-1-AP (DCLK1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 29800-1-AP
IP result of anti-DCLK1 (IP:29800-1-AP, 4ug; Detection:29800-1-AP 1:50000) with mouse brain tissue lysate 1280 ug.
IP result of anti-DCLK1 (IP:29800-1-AP, 4ug; Detection:29800-1-AP 1:50000) with mouse brain tissue lysate 1280 ug.
IHC staining of human colon cancer using 29800-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 29800-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 29800-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 29800-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 29800-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DCLK1 antibody (29800-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DCLK1 antibody (29800-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 29800-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DCLK1 antibody (29800-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DCLK1 antibody (29800-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human colon cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
mouse brain tissue
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:16000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:500-1:2000
Immunofluorescence (IF)-P
IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
29800-1-AP targets DCLK1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
DCLK1 (Serine/threonine-protein kinase DCLK1) is also named as DCAMKL1, DCDC3A, KIAA0369 and belongs to the CAMK Ser/Thr protein kinase family. It is a microtubule-associated kinase that can undergo autophosphorylation and it also has microtubule-polymerizing activity that is independent of its protein kinase activity (PMID: 11124993). It plays a unique role in mitotic spindle integrity during early neurogenesis in radial glial cell proliferation and their radial process stability. DCLK1 is a unique marker for distinguishing tumor stem cells from intestinal normal stem cells (PMID: 23202126). This protein has 4 isoforms produced by alternative splicing with the molecular weight of 82 kDa, 81 kDa, 47 kDa and 48 kDa.
Various lysates were subjected to SDS PAGE followed by western blot with 29800-1-AP (DCLK1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human colon cancer using 29800-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 29800-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 29800-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 29800-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of mouse brain using 29800-1-AP
IP result of anti-DCLK1 (IP:29800-1-AP, 4ug; Detection:29800-1-AP 1:50000) with mouse brain tissue lysate 1280 ug.
IF-P Figures
IF Staining of mouse brain using 29800-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DCLK1 antibody (29800-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 29800-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DCLK1 antibody (29800-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
DCLK1 Polyclonal antibody
Catalog Number
29800-1-AP
Citations
-
Dilutions
WB : 1:2000-1:16000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:500-1:2000 IF-P : 1:50-1:500
Applications
WB, IHC, IF-P, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
