DAX-1 Polyclonal antibody, PBS Only

DAX-1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20849-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of MCF-7 using 20849-1-AP (same clone as 20849-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20849-1-AP (DAX-1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20849-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 20849-1-AP (same clone as 20849-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20849-1-AP (DAX-1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20849-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 20849-1-AP (same clone as 20849-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20849-1-AP (DAX-1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20849-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 20849-1-AP (same clone as 20849-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20849-1-AP (DAX-1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20849-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 20849-1-AP (same clone as 20849-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20849-1-AP (DAX-1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20849-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 20849-1-AP (same clone as 20849-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 20849-1-AP (DAX-1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20849-1-PBS in a different storage buffer formulation.

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Product Information

20849-1-PBS targets DAX-1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DAX-1 fusion protein Ag14416
• Full Name: nuclear receptor subfamily 0, group B, member 1
• Calculated Molecular Weight: 470 aa, 52 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC011564
• Gene Symbol: NR0B1
• Gene ID (NCBI): 190
• RRID: AB_2878751
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.