DAX-1 Polyclonal antibody

DAX-1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20849-1-AP

Synonyms

AHC, AHCH, AHX, DAX 1, DAX1, DSS, GTD, HHG, NR0B1, NROB1, Nuclear receptor DAX 1

---

Validation Data Gallery

• 

  WB analysis of MCF-7 using 20849-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20849-1-AP (DAX-1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 20849-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20849-1-AP (DAX-1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human small intestine using 20849-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20849-1-AP (DAX-1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 20849-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20849-1-AP (DAX-1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 20849-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20849-1-AP (DAX-1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of MCF-7 using 20849-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 20849-1-AP (DAX-1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IHC detected in: human small intestine tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20849-1-AP is DAX-1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DAX-1 fusion protein Ag14416
• Full Name: nuclear receptor subfamily 0, group B, member 1
• Calculated Molecular Weight: 470 aa, 52 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC011564
• Gene Symbol: NR0B1
• Gene ID (NCBI): 190
• RRID: AB_2878751
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.