DARS2 Recombinant antibody, PBS Only (Detector)

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  WB analysis using 85691-2-RR (same clone as 85691-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85691-2-RR (DARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85691-2-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 85691-2-RR (same clone as 85691-2-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 85691-2-RR (DARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85691-2-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 85691-2-RR (same clone as 85691-2-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 85691-2-RR (DARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85691-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02026-2

  Cytometric bead array standard curve of MP02026-2, DARS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85691-3-PBS. Detection antibody: 85691-2-PBS. Standard: Ag4809. Range: 0.781-100 ng/mL.

DARS2 {ptg:Brand} Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 85691-2-PBS

Clone No.243050E5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

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Product Information

85691-2-PBS targets DARS2 as part of a matched antibody pair:

MP02026-2: 85691-3-PBS capture and 85691-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DARS2 fusion protein Ag4809
• Full Name: aspartyl-tRNA synthetase 2, mitochondrial
• Calculated Molecular Weight: 645 aa, 74 kDa
• Observed Molecular Weight: 66-74 kDa
• GenBank Accession Number: BC045173
• Gene Symbol: DARS2
• Gene ID (NCBI): 55157
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6PI48
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

DARS2 is also named as AspRS (aspartyl-tRNA synthetase) and belongs to the class-II aminoacyl-tRNA synthetase family. The deduced 645-amino acid protein has a 47-amino acid mitochondrial targeting signal, resulting in a mature protein of 598 amino acids. DARS2 contains conserved residues involved in ATP binding, tRNA binding, and aspartic acid recognition, as well as catalytic site motifs characteristic of amino acid tRNA synthetases. It is a dimeric proteins(PMID:19443655). Rat aspartyl-tRNA synthetase has a N-terminal polypeptide extension of about 40 amino acid residues which can be removed without impairing its catalytic activity(PMID:9030747). This protein has different molecular mass(55 kDa, 50 kDa,66 kDa ) according to the publications(PMID:11306575; 19443655;15299749).