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  • Western Blot (WB) analysis of various lysates using DARS2 Recombinant antibody (85691-2-RR)

    WB analysis using 85691-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85691-2-RR (DARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using DARS2 Recombinant antibody (85691-2-RR)

    WB analysis of mouse liver using 85691-2-RR

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 85691-2-RR (DARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using DARS2 Recombinant antibody (85691-2-RR)

    WB analysis of Jurkat using 85691-2-RR

    Jurkat cells were subjected to SDS PAGE followed by western blot with 85691-2-RR (DARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

DARS2 Recombinant antibody

DARS2 {ptg:Brand} Recombinant Antibody for WB, ELISA
Cat No. 85691-2-RR
Clone No.243050E5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inMCF-7 cells, Jurkat cells, LNCaP cells, A549 cells, HepG2 cells, NCI-H1299 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85691-2-RR targets DARS2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DARS2 fusion protein Ag4809 Predict reactive species
Full Name aspartyl-tRNA synthetase 2, mitochondrial
Calculated Molecular Weight 645 aa, 74 kDa
Observed Molecular Weight 66-74 kDa
GenBank Accession NumberBC045173
Gene Symbol DARS2
Gene ID (NCBI) 55157
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6PI48
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DARS2 is also named as AspRS (aspartyl-tRNA synthetase) and belongs to the class-II aminoacyl-tRNA synthetase family. The deduced 645-amino acid protein has a 47-amino acid mitochondrial targeting signal, resulting in a mature protein of 598 amino acids. DARS2 contains conserved residues involved in ATP binding, tRNA binding, and aspartic acid recognition, as well as catalytic site motifs characteristic of amino acid tRNA synthetases. It is a dimeric proteins(PMID:19443655). Rat aspartyl-tRNA synthetase has a N-terminal polypeptide extension of about 40 amino acid residues which can be removed without impairing its catalytic activity(PMID:9030747). This protein has different molecular mass(55 kDa, 50 kDa,66 kDa ) according to the publications(PMID:11306575; 19443655;15299749).

Protocols

Product Specific Protocols
WB protocol for DARS2 antibody 85691-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols