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CoraLite® Plus 488-conjugated D2HGDH Monoclonal antibody

D2HGDH Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E9B3

Cat no : CL488-66364

Synonyms

EC:1.1.99.39, D-2-hydroxyglutarate dehydrogenase, mitochondrial, D2HGD, 1E9B3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated D2HGDH Monoclonal an (CL488-66364)

    IF Staining of HepG2 using CL488-66364

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 D2HGDH antibody (CL488-66364, Clone: 1E9B3 ) at dilution of 1:200, CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated D2HGDH Monoclonal an (CL488-66364)

    IF Staining of HepG2 using CL488-66364

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 D2HGDH antibody (CL488-66364, Clone: 1E9B3 ) at dilution of 1:200.

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66364 targets D2HGDH in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen D2HGDH fusion protein Ag5036
Full Name D-2-hydroxyglutarate dehydrogenase
Calculated Molecular Weight 521 aa, 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC036604
Gene Symbol D2HGDH
Gene ID (NCBI) 728294
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

D2HGDH(D-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as D2HGD and belongs to the FAD-binding oxidoreductase/transferase type 4 family.It catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.Defects in D2HGDH are the cause of D-2-hydroxyglutaric aciduria type 1 (D2HGA1).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 D2HGDH antibody CL488-66364Download protocol
Standard Protocols
Click here to view our Standard Protocols