CoraLite®555-conjugated Cytokeratin 7 Monoclonal antibody

• Featured Product
• KD/KO Validated

Cytokeratin 7 Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

2E1G5

Cat no : CL555-66483

Synonyms

KRT7, Cytokeratin-7, Cytokeratin,Keratin, CK-7, CK7

---

Validation Data Gallery

• 

  IF Staining of human prostate cancer using CL555-66483

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite®555 Cytokeratin 7 antibody (CL555-66483, Clone: 2E1G5 ) at dilution of 1:200.

• 

  IF Staining of human prostate cancer using CL555-66483

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite®555 Cytokeratin 7 antibody (CL555-66483, Clone: 2E1G5 ) at dilution of 1:200.

• 

  FC experiment of HeLa using CL555-66483

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug CoraLite®555 Anti-Human Cytokeratin 7 (CL555-66483, Clone:2E1G5) (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive IF-P detected in: human prostate cancer tissue
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66483 targets Cytokeratin 7 in IF-P, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cytokeratin 7 fusion protein Ag7895
• Full Name: keratin 7
• Calculated Molecular Weight: 469 aa, 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC002700
• Gene Symbol: Cytokeratin 7
• Gene ID (NCBI): 3855
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7, also named as cytokeratin 7, is one member of type II basic cytokeratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels, and their neoplasms. KRT7 is marker of epithelial tissues, but not present in carcinomas of stratified squamous cell origin.This antibody is specifically against KRT7.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C