Cytokeratin 5/6 Monoclonal antibody, PBS Only

Cytokeratin 5/6 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F9E1

Cat no : 68295-1-PBS

Synonyms

KRT6B, Cytokeratin 6B, CK-6B, CK6B, CK5/6

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Validation Data Gallery

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  WB analysis using 68295-1-Ig (same clone as 68295-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 68295-1-Ig (same clone as 68295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:2300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 68295-1-Ig (same clone as 68295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:2300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68295-1-Ig (same clone as 68295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:1100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 68295-1-Ig (same clone as 68295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:1100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 68295-1-Ig (same clone as 68295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:2300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 68295-1-Ig (same clone as 68295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:2300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human cervical cancer using 68295-1-Ig (same clone as 68295-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using Cytokeratin 5/6 antibody (68295-1-Ig, Clone: 1F9E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68295-1-Ig (same clone as 68295-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 5/6 antibody (68295-1-Ig, Clone: 1F9E1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

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Product Information

68295-1-PBS targets Cytokeratin 5/6 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cytokeratin 5/6 fusion protein Ag11288
• Full Name: keratin 6B
• Calculated Molecular Weight: 564 aa, 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC034535
• Gene Symbol: Cytokeratin 6B
• Gene ID (NCBI): 3854
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. This antibody can react with Cytokeratin 5 and Cytokeratin 6.