Cytokeratin 5/6 Monoclonal antibody

Cytokeratin 5/6 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1F9E1

Cat no : 68295-1-Ig

Synonyms

KRT6B, Cytokeratin 6B, CK-6B, CK6B, CK5/6

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Validation Data Gallery

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  WB analysis using 68295-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 68295-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:2300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 68295-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:2300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 68295-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:1100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 68295-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:1100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 68295-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:2300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 68295-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:2300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human cervical cancer using 68295-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using Cytokeratin 5/6 antibody (68295-1-Ig, Clone: 1F9E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 68295-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 5/6 antibody (68295-1-Ig, Clone: 1F9E1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HaCaT cells, A431 cells, human saliva, rat skin tissue, mouse skin tissue
• Positive IHC detected in: human cervical cancer tissue, human lung cancer tissue, human oesophagus cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human cervical cancer tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1150-1:4600
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68295-1-Ig targets Cytokeratin 5/6 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cytokeratin 5/6 fusion protein Ag11288
• Full Name: keratin 6B
• Calculated Molecular Weight: 564 aa, 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC034535
• Gene Symbol: Cytokeratin 6B
• Gene ID (NCBI): 3854
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. This antibody can react with Cytokeratin 5 and Cytokeratin 6.