Cytokeratin 17-specific Recombinant antibody

Cytokeratin 17-specific Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242240A8

Cat no : 84978-1-RR

Synonyms

Cytokeratin 17, KRT17, Cytokeratin-17, Cytokeratin,Keratin, CK-17

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Validation Data Gallery

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  WB analysis using 84978-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84978-1-RR (KRT17-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 84978-1-RR

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84978-1-RR (Cytokeratin 17-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 84978-1-RR

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84978-1-RR (Cytokeratin 17-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 84978-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84978-1-RR (Cytokeratin 17-Specific antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 84978-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84978-1-RR (Cytokeratin 17-Specific antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 84978-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 17-Specific antibody (84978-1-RR, Clone: 242240A8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HeLa using 84978-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 17-Specific antibody (84978-1-RR, Clone: 242240A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, mouse skin tissue, rat skin tissue
• Positive IHC detected in: human skin cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84978-1-RR targets Cytokeratin 17-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: keratin 17
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: NM_000422
• Gene Symbol: Cytokeratin 17
• Gene ID (NCBI): 3872
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filaments that make up the cytoskeleton. Type I keratins are a group of acidic intermediate filament proteins and type II keratins are the basic or neutral counterparts. Keratin 17 is encoded by the KRT17 gene and is a type I cytokeratin found in nail beds, hair follicles, sebaceous glands, and epidermal appendages.The molecular weight of cytokeratin 17 is approximately 49 kDa.Cytokeratin 17 plays a role in the formation and maintenance of epidermal appendages, especially in determining the shape and orientation of hair. It is required for the maintenance of the anagen (growth) state of hair follicles by modulating the function of TNF-alpha for hair cycling and is also involved in tissue repair (PMID: 16702408).