Cytokeratin 13 Monoclonal antibody, PBS Only (Capture)

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  WB analysis of A431 using 66684-1-Ig (same clone as 66684-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66684-1-Ig (KRT13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 66684-1-Ig (same clone as 66684-1-PBS)

  WB result of Cytokeratin 13 antibody (66684-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytokeratin 13 transfected A431 cells. This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

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  WB analysis of rat skin using 66684-1-Ig (same clone as 66684-1-PBS)

  rat skin tissue were subjected to SDS PAGE followed by western blot with 66684-1-Ig (KRT13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66684-1-Ig (same clone as 66684-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66684-1-Ig (KRT13 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66684-1-Ig (same clone as 66684-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66684-1-Ig (KRT13 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 66684-1-Ig (same clone as 66684-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66684-1-Ig (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 66684-1-Ig (same clone as 66684-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66684-1-Ig (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 66684-1-Ig (same clone as 66684-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66684-1-Ig (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66684-1-Ig (same clone as 66684-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66684-1-Ig (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 66684-1-Ig (same clone as 66684-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66684-1-Ig (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human cervical cancer using 66684-1-Ig (same clone as 66684-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using Cytokeratin 13 antibody (66684-1-Ig, Clone: 1E10G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51141-1

  Cytometric bead array standard curve of MP51141-1, Cytokeratin 13 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66684-1-PBS. Detection antibody: 66684-2-PBS. Standard:Ag0217. Range: 0.098-100 ng/mL.

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  Cytometric bead array standard curve of MP51141-3

  Cytometric bead array standard curve of MP51141-3, Cytokeratin 13 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66684-1-PBS. Detection antibody: 66684-4-PBS. Standard:Ag0217. Range: 0.098-100 ng/mL.

• Featured Product
• KD/KO Validated

Cytokeratin 13 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Cat No. 66684-1-PBS

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Synonyms

KRT13, Cytokeratin,Keratin, ck17, CK-13, CK13

Formulation:  PBS Only

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Product Information

66684-1-PBS targets Cytokeratin 13 as part of a matched antibody pair:

MP51141-1: 66684-1-PBS capture and 66684-2-PBS detection (validated in Cytometric bead array)

MP51141-3: 66684-1-PBS capture and 66684-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cytokeratin 13 fusion protein Ag0217
• Full Name: keratin 13
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC002661
• Gene Symbol: Cytokeratin 13
• Gene ID (NCBI): 3860
• RRID: AB_2882038
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13646
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. The Keratin 13 (KRT13) gene encodes a type I acidic keratin which is expressed in the differentiated cells of non-cornified stratified squamous epithelia. This antibody can react with Cytokeratin 15.