CoraLite® Plus 488-conjugated Cytokeratin 1-specific Polyclonal antibody

Cytokeratin 1-specific Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16848

Synonyms

67 kDa cytokeratin, CK 1, CK1, Cytokeratin 1, EHK1, Hair alpha protein, K1, keratin 1, KRT1, KRT1 absorb, KRT1A, KRTA, Type II keratin Kb1

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Validation Data Gallery

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  IF Staining of A431 using CL488-16848

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CoraLite® Plus 488 Cytokeratin 1-specific antibody (CL488-16848) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-16848 targets Cytokeratin 1-specific in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: keratin 1
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: NM_006121
• Gene Symbol: KRT1
• Gene ID (NCBI): 3848
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin-1 is type II keratin. It is a specific marker for terminal differentiation in mammalian epidermis. It is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. This antibody is specifically against ketatin I.