Cystatin B Monoclonal antibody, PBS Only

Cystatin B Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G9B8

Cat no : 66812-1-PBS

Synonyms

CSTB, Cystatin-B, CST6, CPI-B, CPI B

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Validation Data Gallery

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  WB analysis of U-937 using 66812-1-Ig (same clone as 66812-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 66812-1-Ig (Cystatin B antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66812-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human saliva using 66812-1-Ig (same clone as 66812-1-PBS)

  human saliva were subjected to SDS PAGE followed by western blot with 66812-1-Ig (Cystatin B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66812-1-PBS in a different storage buffer formulation.

• 

  WB analysis of THP-1 using 66812-1-Ig (same clone as 66812-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66812-1-Ig (Cystatin B antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66812-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 66812-1-Ig (same clone as 66812-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66812-1-Ig (Cystatin B antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66812-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 66812-1-Ig (same clone as 66812-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66812-1-Ig (Cystatin B antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66812-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 66812-1-Ig (same clone as 66812-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Cystatin B antibody (66812-1-Ig, Clone: 2G9B8 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66812-1-PBS in a different storage buffer formulation.

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Product Information

66812-1-PBS targets Cystatin B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cystatin B fusion protein Ag21291
• Full Name: cystatin B (stefin B)
• Calculated Molecular Weight: 11 kDa
• Observed Molecular Weight: 11-14 kDa
• GenBank Accession Number: BC003370
• Gene Symbol: Cystatin B
• Gene ID (NCBI): 1476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Cystatin B (CSTB), a member of the cystatin superfamily protein, is a stefin that functions as an intracellular thiol protease inhibitor and has been thought to play a role in protecting against the proteases leaking from lysosomes. CSTB plays various functions in a variety of diseases, including epithelial ovarian cancer, colon cancer, and myoclonus epilepsy. Evidence indicates that mutations in CSTB are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies.