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Cystatin B Monoclonal antibody

Cystatin B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2G9B8

Cat no : 66812-1-Ig

Synonyms

CSTB, Cystatin-B, CST6, CPI-B, CPI B

Validation Data Gallery

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  • Western Blot (WB) analysis of U-937 cells using Cystatin B Monoclonal antibody (66812-1-Ig)

    WB analysis of U-937 using 66812-1-Ig

    U-937 cells were subjected to SDS PAGE followed by western blot with 66812-1-Ig (Cystatin B antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human saliva using Cystatin B Monoclonal antibody (66812-1-Ig)

    WB analysis of human saliva using 66812-1-Ig

    human saliva were subjected to SDS PAGE followed by western blot with 66812-1-Ig (Cystatin B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using Cystatin B Monoclonal antibody (66812-1-Ig)

    WB analysis of THP-1 using 66812-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 66812-1-Ig (Cystatin B antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Cystatin B Monoclonal antibody (66812-1-Ig)

    IHC staining of human skin cancer using 66812-1-Ig

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66812-1-Ig (Cystatin B antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Cystatin B Monoclonal antibody (66812-1-Ig)

    IHC staining of human skin cancer using 66812-1-Ig

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66812-1-Ig (Cystatin B antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Cystatin B Monoclonal antibody (66812-1-Ig)

    IF Staining of A431 using 66812-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Cystatin B antibody (66812-1-Ig, Clone: 2G9B8 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inU-937 cells, human saliva tissue, THP-1 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66812-1-Ig targets Cystatin B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cystatin B fusion protein Ag21291
Full Name cystatin B (stefin B)
Calculated Molecular Weight 11 kDa
Observed Molecular Weight 11-14 kDa
GenBank Accession NumberBC003370
Gene Symbol Cystatin B
Gene ID (NCBI) 1476
RRIDAB_2882155
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cystatin B (CSTB), a member of the cystatin superfamily protein, is a stefin that functions as an intracellular thiol protease inhibitor and has been thought to play a role in protecting against the proteases leaking from lysosomes. CSTB plays various functions in a variety of diseases, including epithelial ovarian cancer, colon cancer, and myoclonus epilepsy. Evidence indicates that mutations in CSTB are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies.

Protocols

Product Specific Protocols
WB protocol for Cystatin B antibody 66812-1-IgDownload protocol
IHC protocol for Cystatin B antibody 66812-1-IgDownload protocol
IF protocol for Cystatin B antibody 66812-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols