CoraLite® Plus 488-conjugated Cyclophilin B Monoclonal antibody

• Featured Product
• KD/KO Validated

Cyclophilin B Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

8H4F1

Cat no : CL488-66047

Synonyms

PPIB, Peptidyl-prolyl cis-trans isomerase B, EC:5.2.1.8, CYP-S1, CYPB

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Validation Data Gallery

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  WB analysis using CL488-66047

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66047 (Cyclophilin B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using CL488-66047

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66047 (Cyclophilin B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66047 targets Cyclophilin B in WB applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cyclophilin B fusion protein Ag17957
• Full Name: peptidylprolyl isomerase B (cyclophilin B)
• Calculated Molecular Weight: 216 aa, 24 kDa
• Observed Molecular Weight: 19-24 kDa
• GenBank Accession Number: BC032138
• Gene Symbol: Cyclophilin B
• Gene ID (NCBI): 5479
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PPIB, also named as CYPB, SCYLP, PPIase B and CYP-S1, belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. The full-length of PPIB has 216aa (24 kDa), and a 33aa-signal peptide could be removed in the mature form (about 19 kDa).