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Cyclin D2 Monoclonal antibody, PBS Only

Cyclin D2 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A12A8

Cat no : 67048-1-PBS

Synonyms

CCND2, cyclin D, cyclin D2, G1/S specific cyclin D2, KIAK0002

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cyclin D2 Monoclonal antibody (67048-1-Ig)

    WB analysis using 67048-1-Ig (same clone as 67048-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67048-1-Ig (CCND2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67048-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using Cyclin D2 Monoclonal antibody (67048-1-Ig)

    WB analysis of NIH/3T3 using 67048-1-Ig (same clone as 67048-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67048-1-Ig (CCND2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67048-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67048-1-PBS targets Cyclin D2 in WB, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Cyclin D2 fusion protein Ag25190
Full Name cyclin D2
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC010958
Gene Symbol CCND2
Gene ID (NCBI) 894
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CCND2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. CCND2 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. CCND2 has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. High level expression of CCND2 was observed in ovarian and testicular tumors.