Cyclin D2 Monoclonal antibody

Cyclin D2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

1A12A8

Cat no : 67048-1-Ig

Synonyms

CCND2, cyclin D, cyclin D2, G1/S specific cyclin D2, KIAK0002

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Validation Data Gallery

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  WB analysis using 67048-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67048-1-Ig (CCND2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67048-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67048-1-Ig (CCND2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, NIH/3T3 cells, U2OS cells, MCF-7 cells, Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below

Product Information

67048-1-Ig targets Cyclin D2 in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cyclin D2 fusion protein Ag25190
• Full Name: cyclin D2
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC010958
• Gene Symbol: Cyclin D2
• Gene ID (NCBI): 894
• RRID: AB_2882361
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCND2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. CCND2 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. CCND2 has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. High level expression of CCND2 was observed in ovarian and testicular tumors.

Publications

Species	Application	Title
human	WB	Oncogene; USP13 deubiquitinates and stabilizes cyclin D1 to promote gastric cancer cell cycle progression and cell proliferation; Authors - Cunying Ma
human	WB	Technol Cancer Res Treat; The Analysis of Differentially Expressed circRNAs Under the Antiproliferative Effect From 5-Fluorouracil on Osteosarcoma Cells.; Authors - AiJun Huang
human	WB	Cell Death Dis; Long intergenic non-coding RNA 00473 promotes proliferation and migration of gastric cancer via the miR-16-5p/CCND2 axis and by regulating AQP3.; Authors - Shuaishuai Zhuo
human,mouse	WB	Am J Cancer Res; miR-1297 inhibits osteosarcoma cell proliferation and growth by targeting CCND2; Authors - Pan Jiang
human	WB	Int Immunopharmacol; Hydroxypropyl-β-cyclodextrin inhibits the development of triple negative breast cancer by enhancing antitumor immunity; Authors - Mengmeng Zhu
human	WB	Cancer Chemother Pharmacol; Avermectin B1 mediates antitumor activity and induces autophagy in osteosarcoma through the AMPK/ULK1 signaling pathway; Authors - Xiang Fei