Cyclin D1 Recombinant antibody

Cyclin D1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

6B10

Cat no : 82681-1-RR

Synonyms

CCND1, BCL-1 oncogene, BCL-1, B-cell lymphoma 1 protein, B cell lymphoma 1 protein

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Validation Data Gallery

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  WB analysis using 82681-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82681-1-RR (Cyclin D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human oesophagus cancer using 82681-1-RR

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 82681-1-RR (Cyclin D1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 82681-1-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Cyclin D1 antibody (82681-1-RR, Clone: 6B10 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  Affinity and Kinetic Characterization of 82681-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82681-1-RR against Human Cyclin D1 were performed. The affinity constant is 0.153 nM.

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Tested Applications

• Positive WB detected in: HepG2 cells, A431 cells, HCT 116 cells, SW 1990 cells, NIH/3T3 cells, PC-12 cells
• Positive IHC detected in: human oesophagus cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82681-1-RR targets Cyclin D1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Cyclin D1 fusion protein Ag25384
• Full Name: cyclin D1
• Calculated Molecular Weight: 295 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC000076
• Gene Symbol: Cyclin D1
• Gene ID (NCBI): 595
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.