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Cyclin D1 Recombinant antibody, PBS Only (Detector)

Cyclin D1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6B10

Cat no : 82681-1-PBS

Synonyms

CCND1, BCL-1 oncogene, BCL-1, B-cell lymphoma 1 protein, B cell lymphoma 1 protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cyclin D1 Recombinant antibody (82681-1-RR)

    WB analysis using 82681-1-RR (same clone as 82681-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82681-1-RR (Cyclin D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82681-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP80003-1

    Sandwich ELISA standard curve of MP80003-1

    Sandwich ELISA standard curve of MP80003-1, Cyclin D1 Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 68790-1-PBS. Detection antibody: 82681-1-PBS. Standard: Ag25384. Range: 0.188-12 ng/mL.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cyclin D1 Recombinant antibody (82681-1-RR)

    IHC staining of human oesophagus cancer using 82681-1-RR (same clone as 82681-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 82681-1-RR (Cyclin D1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82681-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Cyclin D1 Recombinant antibody (82681-1-RR)

    IF Staining of MCF-7 using 82681-1-RR (same clone as 82681-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Cyclin D1 antibody (82681-1-RR, Clone: 6B10 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82681-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82681-1-RR

    Affinity and Kinetic Characterization of 82681-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82681-1-RR against Human Cyclin D1 were performed. The affinity constant is 0.153 nM.

Product Information

82681-1-PBS targets Cyclin D1 as part of a matched antibody pair:

MP80003-1: 68790-1-PBS capture and 82681-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cyclin D1 fusion protein Ag25384
Full Name cyclin D1
Calculated Molecular Weight 295 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC000076
Gene Symbol Cyclin D1
Gene ID (NCBI) 595
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.