Cxcl15 Recombinant antibody

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  WB analysis of mouse lung using 83245-1-RR

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 83245-1-RR (Cxcl15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83245-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83245-1-RR against Mouse Cxcl15 were performed. The affinity constant is 0.101 nM.

Cxcl15 {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 83245-1-RR

Clone No.240163A6

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Cxcl15, lungkine, Scyb15, weche

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Tested Applications

• Positive WB detected in: mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83245-1-RR targets Cxcl15 in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Cxcl15 fusion protein Ag33964
• Full Name: chemokine (C-X-C motif) ligand 15
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC061138
• Gene Symbol: Cxcl15
• Gene ID (NCBI): 20309
• RRID: AB_3670920
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9WVL7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cxcl15, also known as lungkine or WECHE, is a small cytokine belonging to the CXC chemokine family that has been described in the mouse. High levels of Cxcl15 mRNA were specifically detected in the lung and at lower levels in fetal lung tissue by Northern blot and in situ hybridization, suggesting a potential role for this chemokine during lung development. Moreover, the Cxcl15 protein is secreted into the airway spaces and induces the in vitro and in vivo migration of neutrophils, suggesting that it is involved in lung-specific neutrophil trafficking.