C-Reactive Protein/CRP Recombinant antibody

C-Reactive Protein/CRP Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240369B10

Cat no : 83711-1-RR

Synonyms

Crp, C-reactive protein, C reactive protein, 240369B10

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Validation Data Gallery

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  WB analysis of mouse serum using 83711-1-RR

  mouse serum were subjected to SDS PAGE followed by western blot with 83711-1-RR (Crp antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83711-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83711-1-RR against Mouse Crp were performed. The affinity constant is 0.522 nM.

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Tested Applications

• Positive WB detected in: mouse serum

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83711-1-RR is C-Reactive Protein/CRP Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: C-reactive protein, pentraxin-related
• Observed Molecular Weight: 25-28 kDa
• GenBank Accession Number: NM_007768
• Gene Symbol: Crp
• Gene ID (NCBI): 12944
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein belonging to the pentraxin family. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis, and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation. This antibody is specific for mouse Crp.