Cortactin Recombinant antibody

Cortactin Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241987A12

Cat no : 84569-1-RR

Synonyms

CTTN, SRC8, EMS1, 241987A12

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Validation Data Gallery

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  WB analysis using 84569-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84569-1-RR (Cortactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84569-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84569-1-RR against Human Cortactin were performed. The affinity constant is 7.44 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84569-1-RR targets Cortactin in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Cortactin fusion protein Ag1940
• Full Name: cortactin
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 70-85 kDa
• GenBank Accession Number: BC008799
• Gene Symbol: Cortactin
• Gene ID (NCBI): 2017
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cortactin, encoded by CTTN gene (formerly EMS1), was originally identified as a substrate of the Src kinase. It is a cytoskeletal protein that interacts with F-actin and Arp2/3 complex and plays an important role in regulation of actin assembly and cytoskeleton dynamics. Overexpression of cortactin increases cell migration, and is thought to contribute to the invasive phenotype of breast cancer and several other tumors. Three isoforms of cortactin exist due to the alternative splicing. Although cortactin has a predicted molecular weight around 65 kDa, it was detected as 80/85 doublet or as multiple bands between 75 and 85 kDa on SDS-PAGE (PMID: 18607838, 19089942).