ChromoTek CoraLite® Plus 555-conjugated PD-L1 VHH

CL555-pdlo targets PD-L1 in FC and IF applications and shows reactivity with Human samples.

Host/Type

Alpaca VHH

Reactivity

Human

Applications

FC, IF

Conjugate

CoraLite® Plus 555 Fluorescent Dye

Cat no : CL555-pdlo

Synonyms

PD-L1-Nanobody, PD-L1-Single domain antibody (sdAb), B7 homolog 1, B7-H1, CD274, programmed cell death 1 ligand 1, PDL1, PD-L1

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Validation Data Gallery

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  1X10^6 HEK PD-L1 transient transfected cells (red) and HEK parental cells were surface stained with 0.5 µg CoraLite® Plus 555 conjugated-PD-L1 VHH (CL555-pdlo). Cells were not fixed.

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  Left: 1x10^6 PHA-treated (3d) human PBMCs were surface stained with 0.125ug CoraLite® Plus 555 conjugated PD-L1 VHH (CL555-pdlo) and CL750 CD3 (CL750-65151). Right: 1x10^6 PHA-treated (3d) human PBMCs were surface stained with 0.125ug CoraLite® Plus 555 conjugated PD-L1 VHH (CL555-pdlo) (red) or unstained (blue). 1X10^6 untreated human PBMCs were surface stained with 0.125ug CoraLite® Plus 555 conjugated PD-L1 VHH (CL555-pdlo) (black dashed). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

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  A431 cells transfected with human PD-L1 were immunostained with CoraLite® Plus 555 conjugated-PD-L1 VHH (CL555-pdlo) (1:1000, yellow). Nuclei were stained with DAPI (cyan). Epifluorescence images were acquired with a 20x objective and post-processed. Comment: Immunostaining with CL555-pdlo can be performed live or after formaldehyde fixation.

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Product Information

CL555-pdlo targets PD-L1 in FC and IF applications and shows reactivity with Human samples.

• Applications: FC, IF
• Reactivity: Human
• Conjugate: CoraLite® Plus 555 Fluorescent Dye
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity: Picomolar range, below the assay limit (biolayer interferometry)
• Molecular Weight: 13.887 kDa
• Form: Liquid
• Excitation/Emission Maxima Wavelengths: 554 nm / 570 nm
• RRID: AB_3661835
• Storage Buffer: 500 mM NaCl, 10 mM HEPES pH 7.0, 5 mM EDTA, 0.09% sodium azide
• Storage Condition: Store at -20°C
• Background: PD-L1, also known as CD274 or B7H1, stands for programmed cell death ligand 1. It is a type I transmembrane protein that is thought to repress immune responses by binding to its receptor (PD1), thus inhibiting T-cell activation, proliferation, and cytokine production. It contains V-like and C-like immunoglobulin domains. PD-L1 expression is regulated by various cytokines, such as TNF-α or LPS (ISSN: 1848-7718). Increased expression of this protein in certain types of cancers, e.g., renal cell carcinoma or colon cancer, correlates with poor prognosis. D-L1 is critical for the induction and maintenance of immune self-tolerance during infection or inflammation in normal tissues. The interaction of PD-L1 and its receptors is responsible for preventing auto-immune phenotypes and balancing the overall immune response in situations such as pregnancy or tissue allografts. The interaction between PD-L1 and PD-1 or B7.1 starts an inhibitory signaling cascade, which results in the decreased proliferation of antigen-specific T-cells and increased survival of regulatory T-cells (PMID: 15240681).