CoraLite® Plus 488-conjugated Collagen Type VI Polyclonal antibody

• Featured Product
• KD/KO Validated

Collagen Type VI Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17023

Synonyms

COL6A1, COL6, OPLL, Collagen alpha-1(VI) chain, Collagen alpha 1(VI) chain

---

Validation Data Gallery

• 

  IF Staining of human colon using CL488-17023

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using CoraLite® Plus 488 Collagen Type VI antibody (CL488-17023) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skeletal muscle using CL488-17023

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CoraLite® Plus 488 Collagen Type VI antibody (CL488-17023) at dilution of 1:200.

---

Tested Applications

• Positive IF-P detected in: mouse skeletal muscle tissue, human colon tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-17023 targets Collagen Type VI in IF-P applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Collagen Type VI fusion protein Ag10288
• Full Name: collagen, type VI, alpha 1
• Calculated Molecular Weight: 1028 aa, 109 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC052575
• Gene Symbol: COL6A1
• Gene ID (NCBI): 1291
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.