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CoraLite® Plus 488-conjugated Collagen Type VI Polyclonal antibody
Collagen Type VI Polyclonal Antibody for IF-P
Host / Isotype
Rabbit / IgG
Reactivity
mouse
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-17023
Synonyms
Validation Data Gallery
Tested Applications
Positive IF-P detected in | mouse skeletal muscle tissue, human colon tissue |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-17023 targets Collagen Type VI in IF-P applications and shows reactivity with mouse samples.
Tested Reactivity | mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Collagen Type VI fusion protein Ag10288 |
Full Name | collagen, type VI, alpha 1 |
Calculated Molecular Weight | 1028 aa, 109 kDa |
Observed Molecular Weight | 140 kDa |
GenBank Accession Number | BC052575 |
Gene Symbol | COL6A1 |
Gene ID (NCBI) | 1291 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 Collagen Type VI antibody CL488-17023 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |