Collagen Type III (N-terminal) Recombinant antibody, PBS Only

Collagen Type III (N-terminal) Recombinant Antibody for IF, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2O1

Cat no : 80009-1-PBS

Synonyms

COL3A1, Collagen alpha 1(III) chain, Collagen Type III, Collagen Type III (N-terminal), collagen, type III, alpha 1, EDS4A, FLJ34534

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Validation Data Gallery

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  IHC staining of human colon using 80009-1-RR (same clone as 80009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80009-1-RR (COL3A1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80009-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 80009-1-RR (same clone as 80009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80009-1-RR (COL3A1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80009-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 80009-1-RR (same clone as 80009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80009-1-RR (Collagen Type III (N-terminal) antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80009-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 80009-1-RR (same clone as 80009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80009-1-RR (Collagen Type III (N-terminal) antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80009-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 80009-1-RR (same clone as 80009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80009-1-RR (Collagen Type III (N-terminal) antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80009-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 80009-1-RR (same clone as 80009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80009-1-RR (Collagen Type III (N-terminal) antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80009-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 80009-1-RR (same clone as 80009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80009-1-RR (Collagen Type III (N-terminal) antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80009-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 80009-1-RR (same clone as 80009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80009-1-RR (Collagen Type III (N-terminal) antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80009-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 80009-1-RR (same clone as 80009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80009-1-RR (COL3A1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80009-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 80009-1-RR (same clone as 80009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80009-1-RR (COL3A1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80009-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 80009-1-RR (same clone as 80009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 80009-1-RR (COL3A1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80009-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 80009-1-RR (same clone as 80009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 80009-1-RR (COL3A1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80009-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 80009-1-RR (same clone as 80009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80009-1-RR (COL3A1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80009-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 80009-1-RR (same clone as 80009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80009-1-RR (COL3A1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80009-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon using 80009-1-RR (same clone as 80009-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Collagen Type III (N-terminal) antibody (80009-1-RR, Clone: 2O1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80009-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon using 80009-1-RR (same clone as 80009-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Collagen Type III (N-terminal) antibody (80009-1-RR, Clone: 2O1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80009-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80009-1-PBS targets Collagen Type III (N-terminal) in IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Collagen Type III (N-terminal) fusion protein Ag18658
• Full Name: collagen, type III, alpha 1
• Calculated Molecular Weight: 1466 aa, 139 kDa
• GenBank Accession Number: BC028178
• Gene Symbol: COL3A1
• Gene ID (NCBI): 1281
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Type III collagen is a fibrillar forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341). This antibody was raised against 24-152 aa of prepro α1 (III) chain of human type III procollagen. It can't recognize mouse or rat type III collagen.