Collagen Type III (N-terminal) Recombinant antibody

Collagen Type III (N-terminal) Uni-rAb^TM Recombinant Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2O1

Cat no : 80009-1-RR

Synonyms

COL3A1, COL3, Collagen Type III, Collagen alpha-1(III) chain, Collagen alpha 1(III) chain

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Validation Data Gallery

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  IHC staining of human colon using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80009-1-RR (COL3A1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80009-1-RR (COL3A1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80009-1-RR (Collagen Type III (N-terminal) antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80009-1-RR (Collagen Type III (N-terminal) antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80009-1-RR (Collagen Type III (N-terminal) antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80009-1-RR (Collagen Type III (N-terminal) antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80009-1-RR (Collagen Type III (N-terminal) antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80009-1-RR (Collagen Type III (N-terminal) antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 80009-1-RR (COL3A1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 80009-1-RR (COL3A1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80009-1-RR (COL3A1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80009-1-RR (COL3A1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80009-1-RR (COL3A1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80009-1-RR (COL3A1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon using 80009-1-RR

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Collagen Type III (N-terminal) antibody (80009-1-RR, Clone: 2O1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human colon using 80009-1-RR

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Collagen Type III (N-terminal) antibody (80009-1-RR, Clone: 2O1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human colon using 80009-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Collagen Type III (N-terminal) antibody (80009-1-RR, Clone: 2O1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 80009-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80009-1-RR against Human Collagen Type III (N-terminal) were performed. The affinity constant is 1.78 nM.

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Tested Applications

• Positive IHC detected in: human colon tissue, human colon cancer tissue, human ovary tumor tissue, human pancreas cancer tissue, human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human colon tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

80009-1-RR targets Collagen Type III (N-terminal) in IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Collagen Type III (N-terminal) fusion protein Ag18658
• Full Name: collagen, type III, alpha 1
• Calculated Molecular Weight: 1466 aa, 139 kDa
• GenBank Accession Number: BC028178
• Gene Symbol: COL3A1
• Gene ID (NCBI): 1281
• RRID: AB_2882939
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Type III collagen is a fibrillar forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341). This antibody was raised against 24-152 aa of prepro α1 (III) chain of human type III procollagen. It can't recognize mouse or rat type III collagen.