Collagen Type III (N-terminal) Recombinant antibody

Collagen Type III (N-terminal) Recombinant Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG









Cat no : 80009-1-RR


COL3A1, Collagen alpha 1(III) chain, Collagen Type III, Collagen Type III (N-terminal), collagen, type III, alpha 1, EDS4A, FLJ34534

Tested Applications

Positive IHC detected inhuman colon tissue, human colon cancer tissue, human ovary tumor tissue, human pancreas cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman colon tissue

Recommended dilution

Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80009-1-RR targets Collagen Type III (N-terminal) in IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Collagen Type III (N-terminal) fusion protein Ag18658
Full Name collagen, type III, alpha 1
Calculated Molecular Weight 1466 aa, 139 kDa
GenBank Accession NumberBC028178
Gene Symbol COL3A1
Gene ID (NCBI) 1281
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Type III collagen is a fibrillar forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341). This antibody was raised against 24-152 aa of prepro α1 (III) chain of human type III procollagen. It can't recognize mouse or rat type III collagen.


Product Specific Protocols
IHC protocol for Collagen Type III (N-terminal) antibody 80009-1-RRDownload protocol
IF protocol for Collagen Type III (N-terminal) antibody 80009-1-RRDownload protocol
Standard Protocols
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