Cleaved Caspase 3; p17 Monoclonal antibody, PBS Only

Cleaved Caspase 3; p17 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F7B8

Cat no : 68773-1-PBS

Synonyms

CASP3, Caspase3, Caspase 3, CASP-3, CASP 3

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Validation Data Gallery

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  WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Jurkat using 68773-1-Ig (same clone as 68773-1-PBS)

  IP result of anti-Cleaved Caspase 3; p17 (IP:68773-1-Ig, 4ug; Detection:68773-1-Ig 1:5000) with Staurosporine treated Jurkat cells lysate 720 ug. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 68773-1-Ig (same clone as 68773-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68773-1-Ig (Cleaved Caspase 3; P17 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

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  IHC staining of Jurkat using 68773-1-Ig (same clone as 68773-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 68773-1-Ig (same clone as 68773-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 68773-1-Ig (Cleaved Caspase 3; P17 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 68773-1-Ig (same clone as 68773-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 68773-1-Ig (Cleaved Caspase 3; P17 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

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  IF Staining of BV-2 using 68773-1-Ig (same clone as 68773-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed 50 μM Cisplatin (18 hours) treated BV-2 cells using Cleaved Caspase 3; p17 antibody (68773-1-Ig, Clone: 2F7B8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 68773-1-Ig (same clone as 68773-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed non-treated and 1 μM Staurosporine (1/3/6 hours) treated Jurkat cells using Cleaved Caspase 3; p17 antibody (68773-1-Ig, Clone: 2F7B8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO. RGAM002 ). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

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  IF Staining of HSC-T6 using 68773-1-Ig (same clone as 68773-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed non-treated and 1 μM Staurosporine (1/3/6 hours) treated HSC-T6 cells using Cleaved Caspase 3; p17 antibody (68773-1-Ig, Clone: 2F7B8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

• 

  FC experiment of BV-2 using 68773-1-Ig (same clone as 68773-1-PBS)

  1x10^6 non-treated BV-2 cells (dash lines) and cisplatin treated BV-2 cells (solid lines) were intracellularly stained with 0.2 μg Cleaved Caspase 3; p17 Monoclonal antibody (68773-1-Ig, Clone:2F7B8, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA . This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 68773-1-Ig (same clone as 68773-1-PBS)

  1x10^6 non-treated Jurkat cells (dash lines) and staurosporine treated Jurkat cells (solid lines) were intracellularly stained with 0.1 μg Cleaved Caspase 3; p17 Monoclonal antibody (68773-1-Ig, Clone:2F7B8, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA . This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 68773-1-Ig (same clone as 68773-1-PBS)

  1x10^6 non-treated Jurkat cells (dash lines) and staurosporine treated Jurkat cells (solid lines) were intracellularly stained with 0.2 μg Cleaved Caspase 3; p17 Monoclonal antibody (68773-1-Ig, Clone:2F7B8, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA . This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

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Product Information

68773-1-PBS targets Cleaved Caspase 3; p17 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: caspase 3, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: NM_032991
• Gene Symbol: Caspase 3
• Gene ID (NCBI): 836
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody can recognize p17 of Caspase 3. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3, it can recognize the 17 kDa cleaved-caspase 3. The cleaved P17 and P19 fragment might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826).