Cleaved Caspase 3; p17 Monoclonal antibody
Cleaved Caspase 3; p17 Monoclonal Antibody for WB, IF, ELISA, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human, rat and More (1)
Applications
WB, IF, ELISA, FC (Intra)
Conjugate
Unconjugated
CloneNo.
2F7B8
Cat no : 68773-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, A2780 cells, HepG2 cells, HSC-T6 cells, Ramos cells |
Positive IF detected in | 1 μM Staurosporine (1/3/6 hour) treated Jurkat cells, 1 μM Staurosporine (1/3/6 hour) treated HSC-T6 cells |
Positive FC detected in | 1 μM Staurosporine (3 hours) treated Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF) | IF : 1:500-1:2000 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
Product Information
68773-1-Ig targets Cleaved Caspase 3; p17 in WB, IF, ELISA, FC (Intra) applications and shows reactivity with Human, rat samples.
Tested Reactivity | Human, rat |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | caspase 3, apoptosis-related cysteine peptidase |
Calculated Molecular Weight | 32 kDa |
Observed Molecular Weight | 17 kDa |
GenBank Accession Number | NM_032991 |
Gene Symbol | Caspase 3 |
Gene ID (NCBI) | 836 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody can recognize p17 of Caspase 3. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3, it can recognize the 17 kDa cleaved-caspase 3. The cleaved P17 and P19 fragment might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826).
Protocols
Product Specific Protocols | |
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WB protocol for Cleaved Caspase 3; p17 antibody 68773-1-Ig | Download protocol |
IF protocol for Cleaved Caspase 3; p17 antibody 68773-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Front Nutr Structural Characterization and Anti-breast Cancer Activity in vitro of a Novel Polysaccharide From Cymbopogon citratus |