Chromogranin A Monoclonal antibody, PBS Only (Capture/Detector)

Chromogranin A Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A7D11

Cat no : 60135-2-PBS

Synonyms

CHGA, CGA, EA-92, Chromogranin-A, AL-11

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Validation Data Gallery

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  WB analysis of rat adrenal gland using 60135-2-Ig (same clone as 60135-2-PBS)

  rat adrenal gland tissue were subjected to SDS PAGE followed by western blot with 60135-2-Ig (Chromogranin A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60135-2-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 60135-2-Ig (same clone as 60135-2-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 60135-2-Ig (Chromogranin A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60135-2-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60135-2-Ig (same clone as 60135-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60135-2-Ig (CHGA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60135-2-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60135-2-Ig (same clone as 60135-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60135-2-Ig (CHGA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60135-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60135-2-Ig (same clone as 60135-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60135-2-Ig (CHGA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60135-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 60135-2-Ig (same clone as 60135-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60135-2-Ig (CHGA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60135-2-PBS in a different storage buffer formulation.

• 

  IF Staining of human pancreas cancer using 60135-2-Ig (same clone as 60135-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using Chromogranin A antibody (60135-2-Ig, Clone: 2A7D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60135-2-PBS in a different storage buffer formulation.

• 

  IF Staining of human pancreas cancer using 60135-2-Ig (same clone as 60135-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using Chromogranin A antibody (60135-2-Ig, Clone: 2A7D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60135-2-PBS in a different storage buffer formulation.

• 

  IF Staining of Neuro-2a using 60135-2-Ig (same clone as 60135-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed Neuro-2a cells using Chromogranin A antibody (60135-2-Ig, Clone: 2A7D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60135-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50241-2

  Cytometric bead array standard curve of MP50241-2, CHGA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60135-2-PBS. Detection antibody: 60135-3-PBS. Standard:Ag19780. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP50241-3

  Cytometric bead array standard curve of MP50241-3, CHGA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68851-1-PBS. Detection antibody: 60135-2-PBS. Standard:Ag19780. Range: 1.563-100 ng/mL.

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Product Information

60135-2-PBS targets Chromogranin A as part of a matched antibody pair:

MP50241-2: 60135-2-PBS capture and 60135-3-PBS detection (validated in Cytometric bead array)

MP50241-3: 68851-1-PBS capture and 60135-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Chromogranin A fusion protein Ag0807
• Full Name: chromogranin A (parathyroid secretory protein 1)
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC006459
• Gene Symbol: Chromogranin A
• Gene ID (NCBI): 1113
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.