Chk1 Recombinant antibody

Chk1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2G7

Cat no : 80056-2-RR

Synonyms

CHEK1, CHK1

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Validation Data Gallery

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  WB analysis using 80056-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80056-2-RR (Chk1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293T using 80056-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HEK-293T cells using Chk1 antibody (80056-2-RR, Clone: 2G7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, K-562 cells, MCF-7 cells, NIH/3T3 cells, HSC-T6 cells
• Positive IF/ICC detected in: HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:100-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

80056-2-RR targets Chk1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: CHK1 checkpoint homolog (S. pombe)
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC004202
• Gene Symbol: Chk1
• Gene ID (NCBI): 1111
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.