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WB analysis of Recombinant protein using 67405-1-Ig (same clone as 67405-1-PBS)
100 nL chicken egg were subjected to SDS-PAGE at non-reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. . This data was developed using the same antibody clone with 67405-1-PBS in a different storage buffer formulation.
WB analysis of Recombinant protein using 67405-1-Ig (same clone as 67405-1-PBS)
50 nL chicken egg were subjected to SDS-PAGE at non-reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. . This data was developed using the same antibody clone with 67405-1-PBS in a different storage buffer formulation.
WB analysis of Recombinant protein using 67405-1-Ig (same clone as 67405-1-PBS)
25 nL chicken egg were subjected to SDS-PAGE at non-reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. . This data was developed using the same antibody clone with 67405-1-PBS in a different storage buffer formulation.
WB analysis of Recombinant protein using 67405-1-Ig (same clone as 67405-1-PBS)
12.5 nL chicken egg were subjected to SDS-PAGE at non-reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. . This data was developed using the same antibody clone with 67405-1-PBS in a different storage buffer formulation.
WB analysis of Recombinant protein using 67405-1-Ig (same clone as 67405-1-PBS)
12.5 nL chicken egg were subjected to SDS-PAGE under reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67405-1-PBS in a different storage buffer formulation.

Chicken Chicken IgY Monoclonal antibody, PBS Only

Chicken IgY Monoclonal Antibody for WB, Indirect ELISA

Cat No. 67405-1-PBS

Clone No.1E12G2

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

Chicken

Applications

WB, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

67405-1-PBS targets Chicken IgY in WB, Indirect ELISA applications and shows reactivity with Chicken samples.

Tested Reactivity	Chicken
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	Chicken IgY
Observed Molecular Weight	180 kDa, 70 kDa
Gene Symbol
Gene ID (NCBI)
RRID	AB_2882646
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

IgY (immunoglobulin Y) is a type of immunoglobulin found in birds and reptiles. High concentration of IgY exists in chicken egg yolk. In chickens, IgY is functional same to IgG. IgY antibodies do not cross-react with Fc receptors, mammalian immunoglobulins such as rheumatoid factors and HAMA or activate the complement system thus could reduce false positive results in immunological assays. This antibody was generated from chicken IgY that extracted from chicken eggs. This product recognizes the heavry chain of checken IgY and can detect the contact form of chicken IgY (~180 kDa) and reduced form (~70 kDa).