Chicken IgY Monoclonal antibody
Chicken IgY Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Chicken
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1E12G2
Cat no : 67405-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Chicken IgY, Chicken egg |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
67405-1-Ig targets Chicken IgY in WB, ELISA applications and shows reactivity with Chicken samples.
Tested Reactivity | Chicken |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | Chicken IgY |
Observed Molecular Weight | 180 kDa, 70 kDa |
Gene Symbol | |
Gene ID (NCBI) | |
RRID | AB_2882646 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IgY (immunoglobulin Y) is a type of immunoglobulin found in birds and reptiles. High concentration of IgY exists in chicken egg yolk. In chickens, IgY is functional same to IgG. IgY antibodies do not cross-react with Fc receptors, mammalian immunoglobulins such as rheumatoid factors and HAMA or activate the complement system thus could reduce false positive results in immunological assays. This antibody was generated from chicken IgY that extracted from chicken eggs. This product recognizes the heavry chain of checken IgY and can detect the contact form of chicken IgY (~180 kDa) and reduced form (~70 kDa).
Protocols
Product Specific Protocols | |
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WB protocol for Chicken IgY antibody 67405-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Carbohydr Polym Carboxymethyl chitosan assembled piezoelectric biosensor for rapid and label-free quantification of immunoglobulin Y. |