CoraLite® Plus 488-conjugated Ch-TOG Monoclonal antibody

Ch-TOG Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

1

CloneNo.

1H5G1

Cat no : CL488-67631

Synonyms

ch TOG, CHTOG, CKAP5, FLJ35359, KIAA0097, MSPS, TOG, TOGp

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-67631

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488-conjugated Ch-TOG antibody (CL488-67631, Clone: 1H5G1 ) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL488-67631 targets Ch-TOG in IF/ICC applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Ch-TOG fusion protein Ag28770
• Full Name: cytoskeleton associated protein 5
• Observed Molecular Weight: 220-240 kDa
• GenBank Accession Number: BC120869
• Gene Symbol: Ch-TOG
• Gene ID (NCBI): 9793
• RRID: AB_2919511
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Ch-TOG (colonic hepatic tumor-overexpressed gene), also known as XMAP215 or CKAP5, is a microtubule polymerase which can promotes cytoplasmic microtubule nucleation and elongation. Through interacting with Aurora-A and TACC3, it plays a major role in organizing spindle poles.

Publications

Species	Application	Title
	IF	Science; The mechanism of acentrosomal spindle assembly in human oocytes; Authors - Tianyu Wu