CoraLite® Plus 488-conjugated Ch-TOG Monoclonal antibody

Ch-TOG Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H5G1

Cat no : CL488-67631

Synonyms

ch TOG, CHTOG, CKAP5, FLJ35359, KIAA0097, MSPS, TOG, TOGp



Tested Applications

Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

CL488-67631 targets Ch-TOG in IF applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Ch-TOG fusion protein Ag28770
Full Name cytoskeleton associated protein 5
Observed Molecular Weight 220-240 kDa
GenBank Accession NumberBC120869
Gene Symbol CKAP5
Gene ID (NCBI) 9793
RRIDAB_2919511
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ch-TOG (colonic hepatic tumor-overexpressed gene), also known as XMAP215 or CKAP5, is a microtubule polymerase which can promotes cytoplasmic microtubule nucleation and elongation. Through interacting with Aurora-A and TACC3, it plays a major role in organizing spindle poles.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Ch-TOG antibody CL488-67631Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Science

The mechanism of acentrosomal spindle assembly in human oocytes

Authors - Tianyu Wu